Quantitative evaluation of the genus <i>Bifidobacterium</i> in stool samples of patients with type 1 and 2 diabetes

Abstract Introduction There is evidence of the existence quantitative changes in microbiome, including Bifidobacterium spp., due to some chronic diseases, such as liver cirrhosis, inflammatory bowel obesity, or celiac disease. Materials and Methods We aimed examine number total bacteria present colon patients with type 1 diabetes (T1DM) 2 (T2DM), well healthy subjects. DNA was extracted from patients’ fecal samples then amplified by real-time PCR determine bacteria. Statistical association selected clinical biochemical features examined. Results The mean numbers belonging genus T1DM T2DM were lower compared control group (p = 0.006, p &lt; 0.001 respectively). no statistical differences between all groups 0.397). In group, a significant correlation detected bifidobacteria age (r 0.441, 0.010), alanine aminotransferease 0.022, r −0.11). T2DM, observed triglycerydes 0.001, −0.61). Moreover, we have found negative HBA1, glucose level, −0.35, −0.024, 0.019, Conclusions composition controls. Further studies are needed clarify relationship these elements picture T1DM.